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KMID : 0616120100410010014
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Korean Journal of Pharmacognosy
2010 Volume.41 No. 1 p.14 ~ p.20
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Inhibition of LPS-induced iNOS, COX-2 Expression and Cytokines Production by Fupenjic Acid in Macrophage Cells
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Yun Chang-Hyeon
Lee Kyung-Tae
Shin Ji-Sun
Park Hee-Juhn
Park Jong-Hee
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Abstract
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In this study, we investigated the anti-inflammatory effects of fupenjic acid (FA) isolated from the Potentilla discolor in both RAW 264.7 and mouse primary peritoneal macrophage cells. FA pretreatment significantly inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) productions in the lipopolysaccharide (LPS)-induced RAW 264.7 and mouse primary peritoneal macrophage cells. Consistent with these observations, Western blot and RT-PCR analyses revealed that FA inhibited the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels. In addition, FA reduced the release of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). These results suggest that the down regulation of iNOS and COX-2 expression and TNF-α and IL-6 production by fupenjic acid are responsible for its anti-inflammatory effects.
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KEYWORD
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Fupenjic acid, LPS, Cytokines, iNOS, COX-2
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